differential interference contrast (dic) microscopy Search Results


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Carl Zeiss upright microscope fitted with differential interference contrast (dic) optics using infrared illumination
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Carl Zeiss differential contrast microscopy (dic)
Schematic representation of the steps involved in visualising the internal structures of ovules within cleared cereal pistils. a Plants were examined to identify tillers containing developing spikes. b , c Individual florets were removed from spikes to identify those at anthesis stage, achieved via observation of pollen on fingernails after anther squashing. c , d Whole pistils and anthers were gently removed and placed in fixative, followed by dehydration in an ethanol series and clearing in Hoyer’s solution. e Pistils were transferred to glass slides and covered with glass coverslips. f Samples were examined using <t>DIC</t> <t>microscopy</t> and Zeiss ZEN software
Differential Contrast Microscopy (Dic), supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss differential interference contrast microscopy dic; nomarski optics
Schematic representation of the steps involved in visualising the internal structures of ovules within cleared cereal pistils. a Plants were examined to identify tillers containing developing spikes. b , c Individual florets were removed from spikes to identify those at anthesis stage, achieved via observation of pollen on fingernails after anther squashing. c , d Whole pistils and anthers were gently removed and placed in fixative, followed by dehydration in an ethanol series and clearing in Hoyer’s solution. e Pistils were transferred to glass slides and covered with glass coverslips. f Samples were examined using <t>DIC</t> <t>microscopy</t> and Zeiss ZEN software
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Carl Zeiss axio observer inverted scope with differential interference contrast brightfield microscopy (dic)
Schematic representation of the steps involved in visualising the internal structures of ovules within cleared cereal pistils. a Plants were examined to identify tillers containing developing spikes. b , c Individual florets were removed from spikes to identify those at anthesis stage, achieved via observation of pollen on fingernails after anther squashing. c , d Whole pistils and anthers were gently removed and placed in fixative, followed by dehydration in an ethanol series and clearing in Hoyer’s solution. e Pistils were transferred to glass slides and covered with glass coverslips. f Samples were examined using <t>DIC</t> <t>microscopy</t> and Zeiss ZEN software
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NanoImaging Services Inc dic (differential interferance contrast) microscopy
Schematic representation of the steps involved in visualising the internal structures of ovules within cleared cereal pistils. a Plants were examined to identify tillers containing developing spikes. b , c Individual florets were removed from spikes to identify those at anthesis stage, achieved via observation of pollen on fingernails after anther squashing. c , d Whole pistils and anthers were gently removed and placed in fixative, followed by dehydration in an ethanol series and clearing in Hoyer’s solution. e Pistils were transferred to glass slides and covered with glass coverslips. f Samples were examined using <t>DIC</t> <t>microscopy</t> and Zeiss ZEN software
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Carl Zeiss axiovert microscope by either differential interference contrast microscopy (dic) or fluorescence microscopy
Schematic representation of the steps involved in visualising the internal structures of ovules within cleared cereal pistils. a Plants were examined to identify tillers containing developing spikes. b , c Individual florets were removed from spikes to identify those at anthesis stage, achieved via observation of pollen on fingernails after anther squashing. c , d Whole pistils and anthers were gently removed and placed in fixative, followed by dehydration in an ethanol series and clearing in Hoyer’s solution. e Pistils were transferred to glass slides and covered with glass coverslips. f Samples were examined using <t>DIC</t> <t>microscopy</t> and Zeiss ZEN software
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Blackwell Science Ltd differential-interference-contrast[dic]-microscopy
Schematic representation of the steps involved in visualising the internal structures of ovules within cleared cereal pistils. a Plants were examined to identify tillers containing developing spikes. b , c Individual florets were removed from spikes to identify those at anthesis stage, achieved via observation of pollen on fingernails after anther squashing. c , d Whole pistils and anthers were gently removed and placed in fixative, followed by dehydration in an ethanol series and clearing in Hoyer’s solution. e Pistils were transferred to glass slides and covered with glass coverslips. f Samples were examined using <t>DIC</t> <t>microscopy</t> and Zeiss ZEN software
Differential Interference Contrast[Dic] Microscopy, supplied by Blackwell Science Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Schematic representation of the steps involved in visualising the internal structures of ovules within cleared cereal pistils. a Plants were examined to identify tillers containing developing spikes. b , c Individual florets were removed from spikes to identify those at anthesis stage, achieved via observation of pollen on fingernails after anther squashing. c , d Whole pistils and anthers were gently removed and placed in fixative, followed by dehydration in an ethanol series and clearing in Hoyer’s solution. e Pistils were transferred to glass slides and covered with glass coverslips. f Samples were examined using DIC microscopy and Zeiss ZEN software

Journal: Plant Methods

Article Title: An optimised clearing protocol for the quantitative assessment of sub-epidermal ovule tissues within whole cereal pistils

doi: 10.1186/s13007-017-0217-z

Figure Lengend Snippet: Schematic representation of the steps involved in visualising the internal structures of ovules within cleared cereal pistils. a Plants were examined to identify tillers containing developing spikes. b , c Individual florets were removed from spikes to identify those at anthesis stage, achieved via observation of pollen on fingernails after anther squashing. c , d Whole pistils and anthers were gently removed and placed in fixative, followed by dehydration in an ethanol series and clearing in Hoyer’s solution. e Pistils were transferred to glass slides and covered with glass coverslips. f Samples were examined using DIC microscopy and Zeiss ZEN software

Article Snippet: Pistils were imaged using differential contrast microscopy (DIC) at ×10 magnification with a Zeiss AxioImager M2 equipped with a Nomarski filter.

Techniques: Microscopy, Software